Protein separation & Western blot:
- Staining total proteins on blot membrane with Ponceau S
- Home-made ECL substrate for Western blot imaging
- Western blot stripping
Cell Biology:
- Thawing frozen cell line
- Sub-culture of adherent cell lines
- Freezing cells
- Lentiviruses production
- Lentiviruses concentration
- Infection of cells by lentiviruses
- Viral transduction of cells using ‘spinfection’
- Cell viability determination using crystal violet staining
- Cell cycle analysis by flow cytometry in fixed cells
- Cell cycle analysis and apoptosis determination by flow cytometry in unfixed cells
